Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice

Protease activation mutants of the highly pathogenic avian influenza virus A/FPV/Rostock/34 (H7N1) have been generated that are fully dependent on the presence of trypsin for growth in cell culture. Unlike wild-type virus, the mutants do not induce systemic infection in chicken embryos and show low...

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Autores principales: Wagner, Ralf, Gabriel, Gülsah, Schlesner, Matthias, Alex, Nina, Herwig, Astrid, Werner, Ortrud, Klenk, Hans-Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630491/
https://www.ncbi.nlm.nih.gov/pubmed/26038453
http://dx.doi.org/10.1038/emi2013.7
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author Wagner, Ralf
Gabriel, Gülsah
Schlesner, Matthias
Alex, Nina
Herwig, Astrid
Werner, Ortrud
Klenk, Hans-Dieter
author_facet Wagner, Ralf
Gabriel, Gülsah
Schlesner, Matthias
Alex, Nina
Herwig, Astrid
Werner, Ortrud
Klenk, Hans-Dieter
author_sort Wagner, Ralf
collection PubMed
description Protease activation mutants of the highly pathogenic avian influenza virus A/FPV/Rostock/34 (H7N1) have been generated that are fully dependent on the presence of trypsin for growth in cell culture. Unlike wild-type virus, the mutants do not induce systemic infection in chicken embryos and show low pathogenicity in both chicken embryos and adult chickens. Inactivated vaccines prepared from the mutants protected chickens and mice very efficiently against infection with highly pathogenic wild-type virus in a cross-reactive manner. The potential of these mutants to be used as veterinary and prepandemic vaccines will be discussed.
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spelling pubmed-36304912013-05-13 Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice Wagner, Ralf Gabriel, Gülsah Schlesner, Matthias Alex, Nina Herwig, Astrid Werner, Ortrud Klenk, Hans-Dieter Emerg Microbes Infect Original Article Protease activation mutants of the highly pathogenic avian influenza virus A/FPV/Rostock/34 (H7N1) have been generated that are fully dependent on the presence of trypsin for growth in cell culture. Unlike wild-type virus, the mutants do not induce systemic infection in chicken embryos and show low pathogenicity in both chicken embryos and adult chickens. Inactivated vaccines prepared from the mutants protected chickens and mice very efficiently against infection with highly pathogenic wild-type virus in a cross-reactive manner. The potential of these mutants to be used as veterinary and prepandemic vaccines will be discussed. Nature Publishing Group 2013-02 2013-02-27 /pmc/articles/PMC3630491/ /pubmed/26038453 http://dx.doi.org/10.1038/emi2013.7 Text en Copyright © 2013 Shanghai Shangyixun Cultural Communication Co., Ltd http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Wagner, Ralf
Gabriel, Gülsah
Schlesner, Matthias
Alex, Nina
Herwig, Astrid
Werner, Ortrud
Klenk, Hans-Dieter
Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice
title Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice
title_full Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice
title_fullStr Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice
title_full_unstemmed Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice
title_short Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice
title_sort protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype h7 in chickens and mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630491/
https://www.ncbi.nlm.nih.gov/pubmed/26038453
http://dx.doi.org/10.1038/emi2013.7
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