Cargando…

Dual fluorescent molecular substrates selectively report the activation, sustainability and reversibility of cellular PKB/Akt activity

Using a newly developed near-infrared (NIR) dye that fluoresces at two different wavelengths (dichromic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kinase B, and a method to quantitatively report this enzyme's activity in real time. Upon insulin...

Descripción completa

Detalles Bibliográficos
Autores principales:	Shen, Duanwen, Bai, Mingfeng, Tang, Rui, Xu, Baogang, Ju, Xiaoming, Pestell, Richard G., Achilefu, Samuel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3631771/ https://www.ncbi.nlm.nih.gov/pubmed/23603888 http://dx.doi.org/10.1038/srep01697

Ejemplares similares

CDKL3 promotes osteosarcoma progression by activating Akt/PKB
por: He, Aina, et al.
Publicado: (2020)

Correction: CDKL3 promotes osteosarcoma progression by activating Akt/PKB
por: He, Aina, et al.
Publicado: (2022)

Mechanism of Activation of PKB/Akt by the Protein Phosphatase Inhibitor Calyculin A
por: Pozuelo-Rubio, Mercedes, et al.
Publicado: (2010)

Akt/PKB Controls the Activity-Dependent Bulk Endocytosis of Synaptic Vesicles
por: Smillie, Karen J, et al.
Publicado: (2012)

Tunable Ultrasmall Visible-to-Extended Near-Infrared Emitting Silver Sulfide Quantum Dots for Integrin-Targeted Cancer Imaging
por: Tang, Rui, et al.
Publicado: (2015)

Akt/PKB suppresses DNA damage processing and checkpoint activation in late G2
por: Xu, Naihan, et al.
Publicado: (2010)

Ack1 Mediated AKT/PKB Tyrosine 176 Phosphorylation Regulates Its Activation
por: Mahajan, Kiran, et al.
Publicado: (2010)

Deletion of PKBα/Akt1 Affects Thymic Development
por: Fayard, Elisabeth, et al.
Publicado: (2007)

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation
por: Chen, Ke, et al.
Publicado: (2020)

Activation of AKT/PKB in breast cancer predicts a worse outcome among endocrine treated patients
por: Pérez-Tenorio, G, et al.
Publicado: (2002)

A Gammaherpesvirus Complement Regulatory Protein Promotes Initiation of Infection by Activation of Protein Kinase Akt/PKB
por: Steer, Beatrix, et al.
Publicado: (2010)

Differential Mechanisms of Constitutive Akt/PKB Activation and Its Influence on Gene Expression in Pancreatic Cancer Cells
por: Matsumoto, Joe, et al.
Publicado: (2002)

The membrane-associated form of cyclin D1 enhances cellular invasion
por: Chen, Ke, et al.
Publicado: (2020)

Oligonucleotides suppress PKB/Akt and act as superinductors of apoptosis in human keratinocytes
por: Kippenberger, Stefan, et al.
Publicado: (2009)

Akt/PKB activation and insulin signaling: a novel insulin signaling pathway in the treatment of type 2 diabetes
por: Mackenzie, Richard WA, et al.
Publicado: (2014)

CTLA-4 Activation of Phosphatidylinositol 3-Kinase (PI 3-K) and Protein Kinase B (PKB/AKT) Sustains T-Cell Anergy without Cell Death
por: Schneider, Helga, et al.
Publicado: (2008)

Activated AKT/PKB signaling in C. elegans uncouples temporally distinct outputs of DAF-2/insulin-like signaling
por: Gami, Minaxi S, et al.
Publicado: (2006)

Correction: CTLA-4 Activation of Phosphatidylinositol 3-Kinase (PI 3-K) and Protein Kinase B (PKB/AKT) Sustains T-Cell Anergy without Cell Death
por: Schneider, Helga, et al.
Publicado: (2009)

Conditional drug screening shows that mitotic inhibitors induce AKT/PKB-insensitive apoptosis
por: Berndtsson, Maria, et al.
Publicado: (2009)

SILAM for quantitative proteomics of liver Akt1/PKBα after burn injury
por: LU, X.-M., et al.
Publicado: (2012)

Ras-mediated activation of the TORC2–PKB pathway is critical for chemotaxis
por: Cai, Huaqing, et al.
Publicado: (2010)

Nitric oxide activates intradomain disulfide bond formation in the kinase loop of Akt1/PKBα after burn injury
por: LU, X.-M., et al.
Publicado: (2013)

p27(KIP1 )phosphorylation by PKB/Akt leads to poor breast cancer prognosis
por: Clarke, Robert B
Publicado: (2003)

Dopamine D2 receptor-mediated Akt/PKB signalling: initiation by the D2S receptor and role in quinpirole-induced behavioural activation
por: Chen, Han-Ting, et al.
Publicado: (2012)

Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose
por: Kim, Mee-Young, et al.
Publicado: (2014)

Molecular alterations in apoptotic pathways after PKB/Akt-mediated chemoresistance in NCI H460 cells
por: Hövelmann, S, et al.
Publicado: (2004)

Two Membrane-Associated Tyrosine Phosphatase Homologs Potentiate C. elegans AKT-1/PKB Signaling
por: Hu, Patrick J, et al.
Publicado: (2006)

Regulation of skeletal muscle growth by the IGF1-Akt/PKB pathway: insights from genetic models
por: Schiaffino, Stefano, et al.
Publicado: (2011)

mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4
por: Castets, Perrine, et al.
Publicado: (2019)

The high‐risk HPV E6 proteins modify the activity of the eIF4E protein via the MEK/ERK and AKT/PKB pathways
por: Morales‐Garcia, Vicente, et al.
Publicado: (2020)

Efficient and cost effective production of active-form human PKB using silkworm larvae
por: Maesaki, Ryoko, et al.
Publicado: (2014)

Lipid phosphatase SHIP2 functions as oncogene in colorectal cancer by regulating PKB activation
por: Hoekstra, Elmer, et al.
Publicado: (2016)

The Masticatory Contractile Load Induced Expression and Activation of Akt1/PKBα in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit
por: Korkmaz, Yüksel, et al.
Publicado: (2010)

Correlation between PKB/Akt, GSK-3β expression and tubular epithelial-mesenchymal transition in renal allografts with chronic active antibody-mediated rejection
por: Yan, Qiang, et al.
Publicado: (2017)

Role of a Novel PH-Kinase Domain Interface in PKB/Akt Regulation: Structural Mechanism for Allosteric Inhibition
por: Calleja, Véronique, et al.
Publicado: (2009)

p32 promotes melanoma progression and metastasis by targeting EMT markers, Akt/PKB pathway, and tumor microenvironment
por: Sinha, Sunita, et al.
Publicado: (2021)

PKBγ/AKT3 loss-of-function causes learning and memory deficits and deregulation of AKT/mTORC2 signaling: Relevance for schizophrenia
por: Howell, Kristy R., et al.
Publicado: (2017)

Polycystic ovarian syndrome: clarifying role of PKBβ
Publicado: (2012)

Synthesis of N-(4-chlorophenyl) substituted pyrano[2,3-c]pyrazoles enabling PKBβ/AKT2 inhibitory and in vitro anti-glioma activity
por: Vala, Ruturajsinh M., et al.
Publicado: (2022)

SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation
por: Constantinou, Thecla, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_jpv75f2m882vkfqefpv4mtbg4e