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Physiological temperature during brain slicing enhances the quality of acute slice preparations
We demonstrate that brain dissection and slicing using solutions warmed to near-physiological temperature (~ +34°C), greatly enhance slice quality without affecting intrinsic electrophysiological properties of the neurons. Improved slice quality is seen not only when using young (<1 month), but a...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632751/ https://www.ncbi.nlm.nih.gov/pubmed/23630465 http://dx.doi.org/10.3389/fncel.2013.00048 |