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Physiological temperature during brain slicing enhances the quality of acute slice preparations

We demonstrate that brain dissection and slicing using solutions warmed to near-physiological temperature (~ +34°C), greatly enhance slice quality without affecting intrinsic electrophysiological properties of the neurons. Improved slice quality is seen not only when using young (<1 month), but a...

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Detalles Bibliográficos
Autores principales: Huang, Shiwei, Uusisaari, Marylka Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632751/
https://www.ncbi.nlm.nih.gov/pubmed/23630465
http://dx.doi.org/10.3389/fncel.2013.00048