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Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae

The Saccharomyces cerevisiae high-affinity phosphate transporter Pho89 is a member of the inorganic phosphate (Pi) transporter (PiT) family, and shares significant homology with the type III Na(+)/Pi symporters, hPit1 and hPit2. Currently, detailed biochemical and biophysical analyses of Pho89 to be...

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Detalles Bibliográficos
Autores principales: Sengottaiyan, Palanivelu, Ruiz-Pavón, Lorena, Persson, Bengt L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633241/
https://www.ncbi.nlm.nih.gov/pubmed/23216645
http://dx.doi.org/10.1111/febs.12090