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Prokaryotic Ubiquitin-Like ThiS Fusion Enhances the Heterologous Protein Overexpression and Aggregation in Escherichia coli

Fusion tags are commonly employed to enhance target protein expression, improve their folding and solubility, and reduce protein degradation in expression of recombinant proteins. Ubiquitin (Ub) and SUMO are highly conserved small proteins in eukaryotes, and frequently used as fusion tags in prokary...

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Detalles Bibliográficos
Autores principales: Yuan, Sujuan, Xu, Jian, Ge, Ying, Yan, Zheng, Du, Guohua, Wang, Nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636215/
https://www.ncbi.nlm.nih.gov/pubmed/23638106
http://dx.doi.org/10.1371/journal.pone.0062529