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Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification

BACKGROUND: Next-generation-sequencing (NGS) technologies combined with a classic DNA barcoding approach have enabled fast and credible measurement for biodiversity of mixed environmental samples. However, the PCR amplification involved in nearly all existing NGS protocols inevitably introduces taxo...

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Detalles Bibliográficos
Autores principales: Zhou, Xin, Li, Yiyuan, Liu, Shanlin, Yang, Qing, Su, Xu, Zhou, Lili, Tang, Min, Fu, Ribei, Li, Jiguang, Huang, Quanfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637469/
https://www.ncbi.nlm.nih.gov/pubmed/23587339
http://dx.doi.org/10.1186/2047-217X-2-4