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Versatile synthesis of probes for high-throughput enzyme activity screening

Mass spectrometry based technologies are promising as generalizable high-throughput assays for enzymatic activity. In one such technology, a specialized enzyme substrate probe is presented to a biological mixture potentially exhibiting enzymatic activity, followed by an in situ enrichment step using...

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Detalles Bibliográficos
Autores principales: de Rond, Tristan, Peralta-Yahya, Pamela, Cheng, Xiaoliang, Northen, Trent R., Keasling, Jay D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637890/
https://www.ncbi.nlm.nih.gov/pubmed/23568610
http://dx.doi.org/10.1007/s00216-013-6888-z