Quantitative Transcriptomics using Designed Primer-based Amplification

We developed a novel Designed Primer-based RNA-sequencing strategy (DP-seq) that uses a defined set of heptamer primers to amplify the majority of expressed transcripts from limiting amounts of mRNA, while preserving their relative abundance. Our strategy reproducibly yielded high levels of amplific...

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Detalles Bibliográficos
Autores principales: Bhargava, Vipul, Ko, Pang, Willems, Erik, Mercola, Mark, Subramaniam, Shankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638165/
https://www.ncbi.nlm.nih.gov/pubmed/23624976
http://dx.doi.org/10.1038/srep01740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638165/
https://www.ncbi.nlm.nih.gov/pubmed/23624976
http://dx.doi.org/10.1038/srep01740

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