A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis

Osteomyelitis therapy is a long-term and inconvenient procedure for a patient. Antibiotic-loaded bone cements are both a complementary and alternative treatment option to intravenous antibiotic therapy for the treatment of osteomyelitis. In the current study, the biphasic calcium phosphate cement (C...

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Autores principales: Su, Wen-Yu, Chen, Yu-Chun, Lin, Feng-Huei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638604/
https://www.ncbi.nlm.nih.gov/pubmed/23662153
http://dx.doi.org/10.1155/2013/801374
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author Su, Wen-Yu
Chen, Yu-Chun
Lin, Feng-Huei
author_facet Su, Wen-Yu
Chen, Yu-Chun
Lin, Feng-Huei
author_sort Su, Wen-Yu
collection PubMed
description Osteomyelitis therapy is a long-term and inconvenient procedure for a patient. Antibiotic-loaded bone cements are both a complementary and alternative treatment option to intravenous antibiotic therapy for the treatment of osteomyelitis. In the current study, the biphasic calcium phosphate cement (CPC), called α-TCP/HAP (α-tricalcium phosphate/hydroxyapatite) biphasic cement, was prepared as an antibiotics carrier for osteomyelitis. The developed biphasic cement with a microstructure of α-TCP surrounding the HAP has a fast setting time which will fulfill the clinical demand. The X-ray diffraction and Fourier transform infrared spectrometry analyses showed the final phase to be HAP, the basic bone mineral, after setting for a period of time. Scanning electron microscopy revealed a porous structure with particle sizes of a few micrometers. The addition of gentamicin in α-TCP/HAP would delay the transition of α-TCP but would not change the final-phase HAP. The gentamicin-loaded α-TCP/HAP supplies high doses of the antibiotic during the initial 24 hours when they are soaked in phosphate buffer solution (PBS). Thereafter, a slower drug release is produced, supplying minimum inhibitory concentration until the end of the experiment (30 days). Studies of growth inhibition of Staphylococcus aureus and Pseudomonas aeruginosa in culture indicated that gentamicin released after 30 days from α-TCP/HAP biphasic cement retained antibacterial activity.
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spelling pubmed-36386042013-05-09 A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis Su, Wen-Yu Chen, Yu-Chun Lin, Feng-Huei Evid Based Complement Alternat Med Research Article Osteomyelitis therapy is a long-term and inconvenient procedure for a patient. Antibiotic-loaded bone cements are both a complementary and alternative treatment option to intravenous antibiotic therapy for the treatment of osteomyelitis. In the current study, the biphasic calcium phosphate cement (CPC), called α-TCP/HAP (α-tricalcium phosphate/hydroxyapatite) biphasic cement, was prepared as an antibiotics carrier for osteomyelitis. The developed biphasic cement with a microstructure of α-TCP surrounding the HAP has a fast setting time which will fulfill the clinical demand. The X-ray diffraction and Fourier transform infrared spectrometry analyses showed the final phase to be HAP, the basic bone mineral, after setting for a period of time. Scanning electron microscopy revealed a porous structure with particle sizes of a few micrometers. The addition of gentamicin in α-TCP/HAP would delay the transition of α-TCP but would not change the final-phase HAP. The gentamicin-loaded α-TCP/HAP supplies high doses of the antibiotic during the initial 24 hours when they are soaked in phosphate buffer solution (PBS). Thereafter, a slower drug release is produced, supplying minimum inhibitory concentration until the end of the experiment (30 days). Studies of growth inhibition of Staphylococcus aureus and Pseudomonas aeruginosa in culture indicated that gentamicin released after 30 days from α-TCP/HAP biphasic cement retained antibacterial activity. Hindawi Publishing Corporation 2013 2013-04-09 /pmc/articles/PMC3638604/ /pubmed/23662153 http://dx.doi.org/10.1155/2013/801374 Text en Copyright © 2013 Wen-Yu Su et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Su, Wen-Yu
Chen, Yu-Chun
Lin, Feng-Huei
A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis
title A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis
title_full A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis
title_fullStr A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis
title_full_unstemmed A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis
title_short A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis
title_sort new type of biphasic calcium phosphate cement as a gentamicin carrier for osteomyelitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638604/
https://www.ncbi.nlm.nih.gov/pubmed/23662153
http://dx.doi.org/10.1155/2013/801374
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