Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant

Complementation for virulence of a non-polar virB5 mutant in Brucella suis 1330 was not possible using a pBBR-based plasmid but was with low copy vector pGL10. Presence of the pBBR-based replicon in wildtype B. suis had a dominant negative effect, leading to complete attenuation in J774 macrophages....

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Detalles Bibliográficos
Autores principales: Sprynski, Nicolas, Felix, Christine, O’Callaghan, David, Vergunst, Annette C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642115/
https://www.ncbi.nlm.nih.gov/pubmed/23650582
http://dx.doi.org/10.1016/j.fob.2012.03.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642115/
https://www.ncbi.nlm.nih.gov/pubmed/23650582
http://dx.doi.org/10.1016/j.fob.2012.03.003

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