Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant

Complementation for virulence of a non-polar virB5 mutant in Brucella suis 1330 was not possible using a pBBR-based plasmid but was with low copy vector pGL10. Presence of the pBBR-based replicon in wildtype B. suis had a dominant negative effect, leading to complete attenuation in J774 macrophages....

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Autores principales: Sprynski, Nicolas, Felix, Christine, O’Callaghan, David, Vergunst, Annette C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642115/
https://www.ncbi.nlm.nih.gov/pubmed/23650582
http://dx.doi.org/10.1016/j.fob.2012.03.003
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author Sprynski, Nicolas
Felix, Christine
O’Callaghan, David
Vergunst, Annette C.
author_facet Sprynski, Nicolas
Felix, Christine
O’Callaghan, David
Vergunst, Annette C.
author_sort Sprynski, Nicolas
collection PubMed
description Complementation for virulence of a non-polar virB5 mutant in Brucella suis 1330 was not possible using a pBBR-based plasmid but was with low copy vector pGL10. Presence of the pBBR-based replicon in wildtype B. suis had a dominant negative effect, leading to complete attenuation in J774 macrophages. This was due to pleiotropic effects on VirB protein expression due to multiple copies of the virB promoter region and over expression of VirB5. Functional complementation of mutants in individual components of multiprotein complexes such as bacterial secretion systems, are often problematic; this study highlights the importance of using a low copy vector.
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spelling pubmed-36421152013-05-06 Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant Sprynski, Nicolas Felix, Christine O’Callaghan, David Vergunst, Annette C. FEBS Open Bio Article Complementation for virulence of a non-polar virB5 mutant in Brucella suis 1330 was not possible using a pBBR-based plasmid but was with low copy vector pGL10. Presence of the pBBR-based replicon in wildtype B. suis had a dominant negative effect, leading to complete attenuation in J774 macrophages. This was due to pleiotropic effects on VirB protein expression due to multiple copies of the virB promoter region and over expression of VirB5. Functional complementation of mutants in individual components of multiprotein complexes such as bacterial secretion systems, are often problematic; this study highlights the importance of using a low copy vector. Elsevier 2012-04-09 /pmc/articles/PMC3642115/ /pubmed/23650582 http://dx.doi.org/10.1016/j.fob.2012.03.003 Text en © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical Societies. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non- commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Sprynski, Nicolas
Felix, Christine
O’Callaghan, David
Vergunst, Annette C.
Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
title Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
title_full Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
title_fullStr Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
title_full_unstemmed Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
title_short Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
title_sort restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a brucella virb5 mutant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642115/
https://www.ncbi.nlm.nih.gov/pubmed/23650582
http://dx.doi.org/10.1016/j.fob.2012.03.003
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