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Cloning, Expression and Characterization of 3-Hydroxyisobutyrate Dehydrogenase from Pseudomonas denitrificans ATCC 13867

The gene encoding an NAD(+)-dependent, 3-hydroxyisobutyrate dehydrogenase (3HIBDH-IV) from Pseudomonas denitrificans ATCC 13867 was cloned and expressed in Escherichia coli BL 21 (DE3) and characterized to understand its physiological relevance in the degradation of 3-hydroxypropionic acid (3-HP). T...

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Detalles Bibliográficos
Autores principales: Zhou, Shengfang, Mohan Raj, Subramanian, Ashok, Somasundar, Edwardraja, Selvakumar, Lee, Sun-gu, Park, Sunghoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642240/
https://www.ncbi.nlm.nih.gov/pubmed/23658760
http://dx.doi.org/10.1371/journal.pone.0062666