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Artifactual pyrosequencing reads in multiple-displacement-amplified sediment metagenomes from the Red Sea

The Multiple Displacement Amplification (MDA) protocol is reported to introduce different artifacts into DNA samples with impurities. In this study, we report an artifactual effect of MDA with sediment DNA samples from a deep-sea brine basin in the Red Sea. In the metagenomes, we showed the presence...

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Detalles Bibliográficos
Autores principales: Wang, Yong, Lee, On On, Yang, Jiang Ke, Li, Tie Gang, Qian, Pei Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642703/
https://www.ncbi.nlm.nih.gov/pubmed/23646288
http://dx.doi.org/10.7717/peerj.69