Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD

The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the oligomeric state of the active helicase complex...

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Detalles Bibliográficos
Autores principales: Chisty, Liisa T., Toseland, Christopher P., Fili, Natalia, Mashanov, Gregory I., Dillingham, Mark S., Molloy, Justin E., Webb, Martin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643603/
https://www.ncbi.nlm.nih.gov/pubmed/23535146
http://dx.doi.org/10.1093/nar/gkt194

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643603/
https://www.ncbi.nlm.nih.gov/pubmed/23535146
http://dx.doi.org/10.1093/nar/gkt194

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