SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites

BACKGROUND: Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert. DpnI digestion of the reaction mixture is used to eli...

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Detalles Bibliográficos
Autores principales: Karnik, Abhijit, Karnik, Rucha, Grefen, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644487/
https://www.ncbi.nlm.nih.gov/pubmed/23522286
http://dx.doi.org/10.1186/1471-2105-14-105

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644487/
https://www.ncbi.nlm.nih.gov/pubmed/23522286
http://dx.doi.org/10.1186/1471-2105-14-105

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