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SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites
BACKGROUND: Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert. DpnI digestion of the reaction mixture is used to eli...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644487/ https://www.ncbi.nlm.nih.gov/pubmed/23522286 http://dx.doi.org/10.1186/1471-2105-14-105 |