Construction of New Ligation-Independent Cloning Vectors for the Expression and Purification of Recombinant Proteins in Silkworms Using BmNPV Bacmid System

A ligation independent cloning (LIC) system has been developed to facilitate the rapid and high-efficiency cloning of genes in a Bombyx mori expression system. This system was confirmed by the expression of human microsomal triglyceride transfer protein (hMTP) fused with EGFP in silkworm larvae and...

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Detalles Bibliográficos
Autores principales: Kato, Tatsuya, Thompson, James R., Park, Enoch Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651184/
https://www.ncbi.nlm.nih.gov/pubmed/23675518
http://dx.doi.org/10.1371/journal.pone.0064007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651184/
https://www.ncbi.nlm.nih.gov/pubmed/23675518
http://dx.doi.org/10.1371/journal.pone.0064007

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