A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination

BACKGROUND: For the disruption of a target gene in molecular microbiology, unmarked mutagenesis is preferable to marked mutagenesis because the former method raises no concern about the polar effect and leaves no selection marker. In contrast to naturally competent bacteria, there is no useful metho...

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Detalles Bibliográficos
Autores principales: Ishikawa, Masahito, Hori, Katsutoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654948/
https://www.ncbi.nlm.nih.gov/pubmed/23594401
http://dx.doi.org/10.1186/1471-2180-13-86

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654948/
https://www.ncbi.nlm.nih.gov/pubmed/23594401
http://dx.doi.org/10.1186/1471-2180-13-86

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