A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter

BACKGROUND: To isolate over-secretors, we subjected to saturation mutagenesis, a strain of P.pastoris exporting E. coli alkaline phosphatase (EAP) fused to the secretory domain of the yeast α factor pheromone through cellular PHO1/KEX2 secretory processing signals as the α-sec-EAP reporter protein....

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Detalles Bibliográficos
Autores principales: Nallaseth, Ferez S, Anderson, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654994/
https://www.ncbi.nlm.nih.gov/pubmed/23602005
http://dx.doi.org/10.1186/1475-2859-12-36

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654994/
https://www.ncbi.nlm.nih.gov/pubmed/23602005
http://dx.doi.org/10.1186/1475-2859-12-36

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