Transient and Stable Expression of the Neurotensin Receptor NTS1: A Comparison of the Baculovirus-Insect Cell and the T-REx-293 Expression Systems

Nowadays, baculovirus-infected insect cells and tetracycline-inducible mammalian cell lines (T-REx-293) are intensively used for G protein-coupled receptor (GPCR) production for crystallography purposes. Here we constructed a suspension T-REx-293 cell line to stably express an engineered neurotensin...

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Detalles Bibliográficos
Autores principales: Xiao, Su, White, Jim F., Betenbaugh, Michael J., Grisshammer, Reinhard, Shiloach, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656039/
https://www.ncbi.nlm.nih.gov/pubmed/23696845
http://dx.doi.org/10.1371/journal.pone.0063679

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656039/
https://www.ncbi.nlm.nih.gov/pubmed/23696845
http://dx.doi.org/10.1371/journal.pone.0063679

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