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Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor

Maturation/M-phase-promoting factor is the universal inducer of M-phase in eukaryotic cells. It is currently accepted that M-phase-promoting factor is identical to the kinase cyclin B–Cdk1. Here we show that cyclin B–Cdk1 and M-phase-promoting factor are not in fact synonymous. Instead, M-phase-prom...

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Autores principales: Hara, Masatoshi, Abe, Yusuke, Tanaka, Toshiaki, Yamamoto, Takayoshi, Okumura, Eiichi, Kishimoto, Takeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658099/
https://www.ncbi.nlm.nih.gov/pubmed/22968705
http://dx.doi.org/10.1038/ncomms2062
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author Hara, Masatoshi
Abe, Yusuke
Tanaka, Toshiaki
Yamamoto, Takayoshi
Okumura, Eiichi
Kishimoto, Takeo
author_facet Hara, Masatoshi
Abe, Yusuke
Tanaka, Toshiaki
Yamamoto, Takayoshi
Okumura, Eiichi
Kishimoto, Takeo
author_sort Hara, Masatoshi
collection PubMed
description Maturation/M-phase-promoting factor is the universal inducer of M-phase in eukaryotic cells. It is currently accepted that M-phase-promoting factor is identical to the kinase cyclin B–Cdk1. Here we show that cyclin B–Cdk1 and M-phase-promoting factor are not in fact synonymous. Instead, M-phase-promoting factor contains at least two essential components: cyclin B–Cdk1 and another kinase, Greatwall kinase. In the absence of Greatwall kinase, the M-phase-promoting factor is undetectable in oocyte cytoplasm even though cyclin B–Cdk1 is fully active, whereas M-phase-promoting factor activity is restored when Greatwall kinase is added back. Although the excess amount of cyclin B–Cdk1 alone, but not Greatwall kinase alone, can induce nuclear envelope breakdown, spindle assembly is abortive. Addition of Greatwall kinase greatly reduces the amount of cyclin B–Cdk1 required for nuclear envelope breakdown, resulting in formation of the spindle with aligned chromosomes. M-phase-promoting factor is thus a system consisting of one kinase (cyclin B–Cdk1) that directs mitotic entry and a second kinase (Greatwall kinase) that suppresses the protein phosphatase 2A-B55 which opposes cyclin B–Cdk1.
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spelling pubmed-36580992013-05-20 Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor Hara, Masatoshi Abe, Yusuke Tanaka, Toshiaki Yamamoto, Takayoshi Okumura, Eiichi Kishimoto, Takeo Nat Commun Article Maturation/M-phase-promoting factor is the universal inducer of M-phase in eukaryotic cells. It is currently accepted that M-phase-promoting factor is identical to the kinase cyclin B–Cdk1. Here we show that cyclin B–Cdk1 and M-phase-promoting factor are not in fact synonymous. Instead, M-phase-promoting factor contains at least two essential components: cyclin B–Cdk1 and another kinase, Greatwall kinase. In the absence of Greatwall kinase, the M-phase-promoting factor is undetectable in oocyte cytoplasm even though cyclin B–Cdk1 is fully active, whereas M-phase-promoting factor activity is restored when Greatwall kinase is added back. Although the excess amount of cyclin B–Cdk1 alone, but not Greatwall kinase alone, can induce nuclear envelope breakdown, spindle assembly is abortive. Addition of Greatwall kinase greatly reduces the amount of cyclin B–Cdk1 required for nuclear envelope breakdown, resulting in formation of the spindle with aligned chromosomes. M-phase-promoting factor is thus a system consisting of one kinase (cyclin B–Cdk1) that directs mitotic entry and a second kinase (Greatwall kinase) that suppresses the protein phosphatase 2A-B55 which opposes cyclin B–Cdk1. Nature Pub. Group 2012-09-11 /pmc/articles/PMC3658099/ /pubmed/22968705 http://dx.doi.org/10.1038/ncomms2062 Text en Copyright © 2012, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Hara, Masatoshi
Abe, Yusuke
Tanaka, Toshiaki
Yamamoto, Takayoshi
Okumura, Eiichi
Kishimoto, Takeo
Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor
title Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor
title_full Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor
title_fullStr Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor
title_full_unstemmed Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor
title_short Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor
title_sort greatwall kinase and cyclin b-cdk1 are both critical constituents of m-phase-promoting factor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658099/
https://www.ncbi.nlm.nih.gov/pubmed/22968705
http://dx.doi.org/10.1038/ncomms2062
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