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Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack

BACKGROUND: Soybean pathogens and pests reduce grain production worldwide. Biotic interaction cause extensive changes in plant gene expression profile and the data produced by functional genomics studies need validation, usually done by quantitative PCR. Nevertheless, this technique relies on accura...

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Detalles Bibliográficos
Autores principales: Miranda, Vívian de Jesus, Coelho, Roberta Ramos, Viana, Antônio Américo Barbosa, de Oliveira Neto, Osmundo Brilhante, Carneiro, Regina Maria Dechechi Gomes, Rocha, Thales Lima, Grossi de Sa, Maria Fatima, Fragoso, Rodrigo Rocha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660166/
https://www.ncbi.nlm.nih.gov/pubmed/23668315
http://dx.doi.org/10.1186/1756-0500-6-196