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Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation

Fluorescence lifetime measurements can provide quantitative readouts of local fluorophore environment and can be applied to biomolecular interactions via Förster resonant energy transfer (FRET). Fluorescence lifetime imaging (FLIM) can therefore provide a high content analysis (HCA) modality to map...

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Detalles Bibliográficos
Autores principales: Alibhai, Dominic, Kelly, Douglas J, Warren, Sean, Kumar, Sunil, Margineau, Anca, Serwa, Remigiusz A, Thinon, Emmanuelle, Alexandrov, Yuriy, Murray, Edward J, Stuhmeier, Frank, Tate, Edward W, Neil, Mark A A, Dunsby, Chris, French, Paul M W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660788/
https://www.ncbi.nlm.nih.gov/pubmed/23184449
http://dx.doi.org/10.1002/jbio.201200185