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Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1

BACKGROUND: The regulation and function of IgE in healthy individuals and in antigen-naïve animals is not well understood. IL-33 administration increases serum IgE in mice with unknown mechanism. We tested the hypothesis that IL-33 provides an antigen-independent stimulus for IgE production and mast...

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Autores principales: Komai-Koma, M, Brombacher, F, Pushparaj, P N, Arendse, B, McSharry, C, Alexander, J, Chaudhuri, R, Thomson, N C, McKenzie, A N J, McInnes, I, Liew, F Y, Xu, D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660789/
https://www.ncbi.nlm.nih.gov/pubmed/22702477
http://dx.doi.org/10.1111/j.1398-9995.2012.02859.x
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author Komai-Koma, M
Brombacher, F
Pushparaj, P N
Arendse, B
McSharry, C
Alexander, J
Chaudhuri, R
Thomson, N C
McKenzie, A N J
McInnes, I
Liew, F Y
Xu, D
author_facet Komai-Koma, M
Brombacher, F
Pushparaj, P N
Arendse, B
McSharry, C
Alexander, J
Chaudhuri, R
Thomson, N C
McKenzie, A N J
McInnes, I
Liew, F Y
Xu, D
author_sort Komai-Koma, M
collection PubMed
description BACKGROUND: The regulation and function of IgE in healthy individuals and in antigen-naïve animals is not well understood. IL-33 administration increases serum IgE in mice with unknown mechanism. We tested the hypothesis that IL-33 provides an antigen-independent stimulus for IgE production and mast cell degranulation. METHODS: IL-33 was administered to naïve wild-type (WT), nude and ST2(−/−), IL-4(−/−), IL4Rα(−/−) and T-or B-cell-specific IL-4Rα(−/−) mice. IgEand cytokines were quantified by ELISA. T- and B-lymphocyte numbers and CD40L expression were determined by flow cytometry. Anaphylaxis was measured by temperature, mast cell degranulation and histamine release. RESULTS: IL-33 enhanced IgE production in naïve WT, T-IL-4Rα(−/−) but not in ST2(−/−), IL-4(−/−), IL-4Rα(−/−) or B-cell-specific IL-4Rα(−/−) mice, demonstrating IL-33 specificity and IL-4 dependency. Moreover, IL-4 was required for IL-33-induced B-cell proliferation and T-cell CD40L expression, which promotes IgE production. IL-33-induced IL-4 production was mainly from innate cells including mast cells and eosinophils. IL-33 increased mast cell surface IgE and triggered degranulation and systemic anaphylaxis in allergen-naïve WT but not in IL-4Rα(−/−) mice. CONCLUSION: IL-33 amplifies IgE synthesis and triggers anaphylaxis in naïve mice via IL-4, independent of allergen. IL-33 may play an important role in nonatopic allergy and idiopathic anaphylaxis.
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spelling pubmed-36607892013-05-22 Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1 Komai-Koma, M Brombacher, F Pushparaj, P N Arendse, B McSharry, C Alexander, J Chaudhuri, R Thomson, N C McKenzie, A N J McInnes, I Liew, F Y Xu, D Allergy Original Articles BACKGROUND: The regulation and function of IgE in healthy individuals and in antigen-naïve animals is not well understood. IL-33 administration increases serum IgE in mice with unknown mechanism. We tested the hypothesis that IL-33 provides an antigen-independent stimulus for IgE production and mast cell degranulation. METHODS: IL-33 was administered to naïve wild-type (WT), nude and ST2(−/−), IL-4(−/−), IL4Rα(−/−) and T-or B-cell-specific IL-4Rα(−/−) mice. IgEand cytokines were quantified by ELISA. T- and B-lymphocyte numbers and CD40L expression were determined by flow cytometry. Anaphylaxis was measured by temperature, mast cell degranulation and histamine release. RESULTS: IL-33 enhanced IgE production in naïve WT, T-IL-4Rα(−/−) but not in ST2(−/−), IL-4(−/−), IL-4Rα(−/−) or B-cell-specific IL-4Rα(−/−) mice, demonstrating IL-33 specificity and IL-4 dependency. Moreover, IL-4 was required for IL-33-induced B-cell proliferation and T-cell CD40L expression, which promotes IgE production. IL-33-induced IL-4 production was mainly from innate cells including mast cells and eosinophils. IL-33 increased mast cell surface IgE and triggered degranulation and systemic anaphylaxis in allergen-naïve WT but not in IL-4Rα(−/−) mice. CONCLUSION: IL-33 amplifies IgE synthesis and triggers anaphylaxis in naïve mice via IL-4, independent of allergen. IL-33 may play an important role in nonatopic allergy and idiopathic anaphylaxis. Blackwell Publishing Ltd 2012-09 2012-06-15 /pmc/articles/PMC3660789/ /pubmed/22702477 http://dx.doi.org/10.1111/j.1398-9995.2012.02859.x Text en Copyright © 2012 John Wiley & Sons A/S http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
Komai-Koma, M
Brombacher, F
Pushparaj, P N
Arendse, B
McSharry, C
Alexander, J
Chaudhuri, R
Thomson, N C
McKenzie, A N J
McInnes, I
Liew, F Y
Xu, D
Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
title Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
title_full Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
title_fullStr Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
title_full_unstemmed Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
title_short Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
title_sort interleukin-33 amplifies ige synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice1
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660789/
https://www.ncbi.nlm.nih.gov/pubmed/22702477
http://dx.doi.org/10.1111/j.1398-9995.2012.02859.x
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