Cargando…
Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during bacterial i...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661663/ https://www.ncbi.nlm.nih.gov/pubmed/23717478 http://dx.doi.org/10.1371/journal.pone.0063759 |
_version_ | 1782270724668391424 |
---|---|
author | Xiang, Yang Wang, Xuan Yan, Chao Gao, Qian Li, Sheng-An Liu, Jie Zhou, Kaifeng Guo, Xiaolong Lee, Wenhui Zhang, Yun |
author_facet | Xiang, Yang Wang, Xuan Yan, Chao Gao, Qian Li, Sheng-An Liu, Jie Zhou, Kaifeng Guo, Xiaolong Lee, Wenhui Zhang, Yun |
author_sort | Xiang, Yang |
collection | PubMed |
description | It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during bacterial infection. By using a murine peritonitis model, we also found that ATP promotes the fight against bacterial infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against bacterial infection. In the present study, we demonstrated a protective role for ATP during bacterial infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from infections. |
format | Online Article Text |
id | pubmed-3661663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36616632013-05-28 Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome Xiang, Yang Wang, Xuan Yan, Chao Gao, Qian Li, Sheng-An Liu, Jie Zhou, Kaifeng Guo, Xiaolong Lee, Wenhui Zhang, Yun PLoS One Research Article It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during bacterial infection. By using a murine peritonitis model, we also found that ATP promotes the fight against bacterial infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against bacterial infection. In the present study, we demonstrated a protective role for ATP during bacterial infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from infections. Public Library of Science 2013-05-22 /pmc/articles/PMC3661663/ /pubmed/23717478 http://dx.doi.org/10.1371/journal.pone.0063759 Text en © 2013 Xiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xiang, Yang Wang, Xuan Yan, Chao Gao, Qian Li, Sheng-An Liu, Jie Zhou, Kaifeng Guo, Xiaolong Lee, Wenhui Zhang, Yun Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome |
title | Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome |
title_full | Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome |
title_fullStr | Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome |
title_full_unstemmed | Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome |
title_short | Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome |
title_sort | adenosine-5'-triphosphate (atp) protects mice against bacterial infection by activation of the nlrp3 inflammasome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661663/ https://www.ncbi.nlm.nih.gov/pubmed/23717478 http://dx.doi.org/10.1371/journal.pone.0063759 |
work_keys_str_mv | AT xiangyang adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT wangxuan adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT yanchao adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT gaoqian adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT lishengan adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT liujie adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT zhoukaifeng adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT guoxiaolong adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT leewenhui adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome AT zhangyun adenosine5triphosphateatpprotectsmiceagainstbacterialinfectionbyactivationofthenlrp3inflammasome |