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Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome

It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during bacterial i...

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Autores principales: Xiang, Yang, Wang, Xuan, Yan, Chao, Gao, Qian, Li, Sheng-An, Liu, Jie, Zhou, Kaifeng, Guo, Xiaolong, Lee, Wenhui, Zhang, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661663/
https://www.ncbi.nlm.nih.gov/pubmed/23717478
http://dx.doi.org/10.1371/journal.pone.0063759
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author Xiang, Yang
Wang, Xuan
Yan, Chao
Gao, Qian
Li, Sheng-An
Liu, Jie
Zhou, Kaifeng
Guo, Xiaolong
Lee, Wenhui
Zhang, Yun
author_facet Xiang, Yang
Wang, Xuan
Yan, Chao
Gao, Qian
Li, Sheng-An
Liu, Jie
Zhou, Kaifeng
Guo, Xiaolong
Lee, Wenhui
Zhang, Yun
author_sort Xiang, Yang
collection PubMed
description It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during bacterial infection. By using a murine peritonitis model, we also found that ATP promotes the fight against bacterial infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against bacterial infection. In the present study, we demonstrated a protective role for ATP during bacterial infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from infections.
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spelling pubmed-36616632013-05-28 Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome Xiang, Yang Wang, Xuan Yan, Chao Gao, Qian Li, Sheng-An Liu, Jie Zhou, Kaifeng Guo, Xiaolong Lee, Wenhui Zhang, Yun PLoS One Research Article It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during bacterial infection. By using a murine peritonitis model, we also found that ATP promotes the fight against bacterial infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against bacterial infection. In the present study, we demonstrated a protective role for ATP during bacterial infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from infections. Public Library of Science 2013-05-22 /pmc/articles/PMC3661663/ /pubmed/23717478 http://dx.doi.org/10.1371/journal.pone.0063759 Text en © 2013 Xiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xiang, Yang
Wang, Xuan
Yan, Chao
Gao, Qian
Li, Sheng-An
Liu, Jie
Zhou, Kaifeng
Guo, Xiaolong
Lee, Wenhui
Zhang, Yun
Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
title Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
title_full Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
title_fullStr Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
title_full_unstemmed Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
title_short Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome
title_sort adenosine-5'-triphosphate (atp) protects mice against bacterial infection by activation of the nlrp3 inflammasome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661663/
https://www.ncbi.nlm.nih.gov/pubmed/23717478
http://dx.doi.org/10.1371/journal.pone.0063759
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