PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae

BACKGROUND: Saccharomyces cerevisiae is extensively used in bio-industries. However, its genetic engineering to introduce new metabolism pathways can cause unexpected phenotypic alterations. For example, humanisation of the glycosylation pathways is a high priority pharmaceutical industry goal for p...

Descripción completa

Detalles Bibliográficos
Autores principales: Bonnet, Christine, Rigaud, Céline, Chanteclaire, Emilie, Blandais, Claire, Tassy-Freches, Emilie, Arico, Christelle, Javaud, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664073/
https://www.ncbi.nlm.nih.gov/pubmed/23688076
http://dx.doi.org/10.1186/1756-0500-6-201

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664073/
https://www.ncbi.nlm.nih.gov/pubmed/23688076
http://dx.doi.org/10.1186/1756-0500-6-201

Ejemplares similares