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PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae
BACKGROUND: Saccharomyces cerevisiae is extensively used in bio-industries. However, its genetic engineering to introduce new metabolism pathways can cause unexpected phenotypic alterations. For example, humanisation of the glycosylation pathways is a high priority pharmaceutical industry goal for p...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664073/ https://www.ncbi.nlm.nih.gov/pubmed/23688076 http://dx.doi.org/10.1186/1756-0500-6-201 |