Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain

BACKGROUND: In mammals, embryonic neural progenitors as well as adult neural stem cells can be prospectively isolated based on the cell surface expression of prominin-1 (CD133), a plasma membrane glycoprotein. In contrast, characterization of neural progenitors in non-mammalian vertebrates endowed w...

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Autores principales: Jászai, József, Graupner, Sylvi, Tanaka, Elly M., Funk, Richard H. W., Huttner, Wieland B., Brand, Michael, Corbeil, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664558/
https://www.ncbi.nlm.nih.gov/pubmed/23723983
http://dx.doi.org/10.1371/journal.pone.0063457
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author Jászai, József
Graupner, Sylvi
Tanaka, Elly M.
Funk, Richard H. W.
Huttner, Wieland B.
Brand, Michael
Corbeil, Denis
author_facet Jászai, József
Graupner, Sylvi
Tanaka, Elly M.
Funk, Richard H. W.
Huttner, Wieland B.
Brand, Michael
Corbeil, Denis
author_sort Jászai, József
collection PubMed
description BACKGROUND: In mammals, embryonic neural progenitors as well as adult neural stem cells can be prospectively isolated based on the cell surface expression of prominin-1 (CD133), a plasma membrane glycoprotein. In contrast, characterization of neural progenitors in non-mammalian vertebrates endowed with significant constitutive neurogenesis and inherent self-repair ability is hampered by the lack of suitable cell surface markers. Here, we have investigated whether prominin-1–orthologues of the major non-mammalian vertebrate model organisms show any degree of conservation as for their association with neurogenic geminative zones within the central nervous system (CNS) as they do in mammals or associated with activated neural progenitors during provoked neurogenesis in the regenerating CNS. METHODS: We have recently identified prominin-1 orthologues from zebrafish, axolotl and chicken. The spatial distribution of prominin-1–positive cells – in comparison to those of mice – was mapped in the intact brain in these organisms by non-radioactive in situ hybridization combined with detection of proliferating neural progenitors, marked either by proliferating cell nuclear antigen or 5-bromo-deoxyuridine. Furthermore, distribution of prominin-1 transcripts was investigated in the regenerating spinal cord of injured axolotl. RESULTS: Remarkably, a conserved association of prominin-1 with germinative zones of the CNS was uncovered as manifested in a significant co-localization with cell proliferation markers during normal constitutive neurogenesis in all species investigated. Moreover, an enhanced expression of prominin-1 became evident associated with provoked, compensatory neurogenesis during the epimorphic regeneration of the axolotl spinal cord. Interestingly, significant prominin-1–expressing cell populations were also detected at distinct extraventricular (parenchymal) locations in the CNS of all vertebrate species being suggestive of further, non-neurogenic neural function(s). CONCLUSION/INTERPRETATION: Collectively, our work provides the first data set describing a comparative analysis of prominin-1–positive progenitor cells across species establishing a framework for further functional characterization in the context of regeneration.
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spelling pubmed-36645582013-05-30 Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain Jászai, József Graupner, Sylvi Tanaka, Elly M. Funk, Richard H. W. Huttner, Wieland B. Brand, Michael Corbeil, Denis PLoS One Research Article BACKGROUND: In mammals, embryonic neural progenitors as well as adult neural stem cells can be prospectively isolated based on the cell surface expression of prominin-1 (CD133), a plasma membrane glycoprotein. In contrast, characterization of neural progenitors in non-mammalian vertebrates endowed with significant constitutive neurogenesis and inherent self-repair ability is hampered by the lack of suitable cell surface markers. Here, we have investigated whether prominin-1–orthologues of the major non-mammalian vertebrate model organisms show any degree of conservation as for their association with neurogenic geminative zones within the central nervous system (CNS) as they do in mammals or associated with activated neural progenitors during provoked neurogenesis in the regenerating CNS. METHODS: We have recently identified prominin-1 orthologues from zebrafish, axolotl and chicken. The spatial distribution of prominin-1–positive cells – in comparison to those of mice – was mapped in the intact brain in these organisms by non-radioactive in situ hybridization combined with detection of proliferating neural progenitors, marked either by proliferating cell nuclear antigen or 5-bromo-deoxyuridine. Furthermore, distribution of prominin-1 transcripts was investigated in the regenerating spinal cord of injured axolotl. RESULTS: Remarkably, a conserved association of prominin-1 with germinative zones of the CNS was uncovered as manifested in a significant co-localization with cell proliferation markers during normal constitutive neurogenesis in all species investigated. Moreover, an enhanced expression of prominin-1 became evident associated with provoked, compensatory neurogenesis during the epimorphic regeneration of the axolotl spinal cord. Interestingly, significant prominin-1–expressing cell populations were also detected at distinct extraventricular (parenchymal) locations in the CNS of all vertebrate species being suggestive of further, non-neurogenic neural function(s). CONCLUSION/INTERPRETATION: Collectively, our work provides the first data set describing a comparative analysis of prominin-1–positive progenitor cells across species establishing a framework for further functional characterization in the context of regeneration. Public Library of Science 2013-05-27 /pmc/articles/PMC3664558/ /pubmed/23723983 http://dx.doi.org/10.1371/journal.pone.0063457 Text en © 2013 Jászai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jászai, József
Graupner, Sylvi
Tanaka, Elly M.
Funk, Richard H. W.
Huttner, Wieland B.
Brand, Michael
Corbeil, Denis
Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain
title Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain
title_full Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain
title_fullStr Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain
title_full_unstemmed Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain
title_short Spatial Distribution of Prominin-1 (CD133) – Positive Cells within Germinative Zones of the Vertebrate Brain
title_sort spatial distribution of prominin-1 (cd133) – positive cells within germinative zones of the vertebrate brain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664558/
https://www.ncbi.nlm.nih.gov/pubmed/23723983
http://dx.doi.org/10.1371/journal.pone.0063457
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