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Alternative Sigma Factor Over-Expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia coli
BACKGROUND: Heterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways. Development of an effective, general heterologous expression system that can be applied to bioprospecting from metagenomic DNA will enable the disc...
Autores principales: | Stevens, David Cole, Conway, Kyle R., Pearce, Nelson, Villegas-Peñaranda, Luis Roberto, Garza, Anthony G., Boddy, Christopher N. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665592/ https://www.ncbi.nlm.nih.gov/pubmed/23724102 http://dx.doi.org/10.1371/journal.pone.0064858 |
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