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Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Transcription activator-like effector nucleases (TALENs) are novel engineered DNA nucleases, and have been proven to be effective for gene specific targeting in various species. Recently we reported gene disruptions in Xenopus embryos by using TALENs. Here we summarize the protocol that is used in o...

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Detalles Bibliográficos
Autores principales: Lei, Yong, Guo, Xiaogang, Deng, Yi, Chen, Yonglong, Zhao, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665704/
https://www.ncbi.nlm.nih.gov/pubmed/23663889
http://dx.doi.org/10.1186/2045-3701-3-21