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ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA

Aspergilli known as black- and white-koji molds which are used for awamori, shochu, makgeolli and other food and beverage fermentations, are reported in the literature as A. luchuensis, A. awamori, A. kawachii, or A. acidus. In order to elucidate the taxonomic position of these species, available ex...

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Autores principales: Hong, Seung-Beom, Lee, Mina, Kim, Dae-Ho, Varga, Janos, Frisvad, Jens C., Perrone, Giancarlo, Gomi, Katsuya, Yamada, Osamu, Machida, Masayuki, Houbraken, Jos, Samson, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665839/
https://www.ncbi.nlm.nih.gov/pubmed/23723998
http://dx.doi.org/10.1371/journal.pone.0063769
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author Hong, Seung-Beom
Lee, Mina
Kim, Dae-Ho
Varga, Janos
Frisvad, Jens C.
Perrone, Giancarlo
Gomi, Katsuya
Yamada, Osamu
Machida, Masayuki
Houbraken, Jos
Samson, Robert A.
author_facet Hong, Seung-Beom
Lee, Mina
Kim, Dae-Ho
Varga, Janos
Frisvad, Jens C.
Perrone, Giancarlo
Gomi, Katsuya
Yamada, Osamu
Machida, Masayuki
Houbraken, Jos
Samson, Robert A.
author_sort Hong, Seung-Beom
collection PubMed
description Aspergilli known as black- and white-koji molds which are used for awamori, shochu, makgeolli and other food and beverage fermentations, are reported in the literature as A. luchuensis, A. awamori, A. kawachii, or A. acidus. In order to elucidate the taxonomic position of these species, available ex-type cultures were compared based on morphology and molecular characters. A. luchuensis, A. kawachii and A. acidus showed the same banding patterns in RAPD, and the three species had the same rDNA-ITS, β-tubulin and calmodulin sequences and these differed from those of the closely related A. niger and A. tubingensis. Morphologically, the three species are not significantly different from each other or from A. niger and A. tubingensis. It is concluded that A. luchuensis, A. kawachii and A. acidus are the same species, and A. luchuensis is selected as the correct name based on priority. Strains of A. awamori which are stored in National Research Institute of Brewing in Japan, represent A. niger (n = 14) and A. luchuensis (n = 6). The neotype of A. awamori (CBS 557.65 =  NRRL 4948) does not originate from awamori fermentation and it is shown to be identical with the unknown taxon Aspergillus welwitschiae. Extrolite analysis of strains of A. luchuensis showed that they do not produce mycotoxins and therefore can be considered safe for food and beverage fermentations. A. luchuensis is also frequently isolated from meju and nuruk in Korea and Puerh tea in China and the species is probably common in the fermentation environment of East Asia. A re-description of A. luchuensis is provided because the incomplete data in the original literature.
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spelling pubmed-36658392013-05-30 ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA Hong, Seung-Beom Lee, Mina Kim, Dae-Ho Varga, Janos Frisvad, Jens C. Perrone, Giancarlo Gomi, Katsuya Yamada, Osamu Machida, Masayuki Houbraken, Jos Samson, Robert A. PLoS One Research Article Aspergilli known as black- and white-koji molds which are used for awamori, shochu, makgeolli and other food and beverage fermentations, are reported in the literature as A. luchuensis, A. awamori, A. kawachii, or A. acidus. In order to elucidate the taxonomic position of these species, available ex-type cultures were compared based on morphology and molecular characters. A. luchuensis, A. kawachii and A. acidus showed the same banding patterns in RAPD, and the three species had the same rDNA-ITS, β-tubulin and calmodulin sequences and these differed from those of the closely related A. niger and A. tubingensis. Morphologically, the three species are not significantly different from each other or from A. niger and A. tubingensis. It is concluded that A. luchuensis, A. kawachii and A. acidus are the same species, and A. luchuensis is selected as the correct name based on priority. Strains of A. awamori which are stored in National Research Institute of Brewing in Japan, represent A. niger (n = 14) and A. luchuensis (n = 6). The neotype of A. awamori (CBS 557.65 =  NRRL 4948) does not originate from awamori fermentation and it is shown to be identical with the unknown taxon Aspergillus welwitschiae. Extrolite analysis of strains of A. luchuensis showed that they do not produce mycotoxins and therefore can be considered safe for food and beverage fermentations. A. luchuensis is also frequently isolated from meju and nuruk in Korea and Puerh tea in China and the species is probably common in the fermentation environment of East Asia. A re-description of A. luchuensis is provided because the incomplete data in the original literature. Public Library of Science 2013-05-28 /pmc/articles/PMC3665839/ /pubmed/23723998 http://dx.doi.org/10.1371/journal.pone.0063769 Text en © 2013 Hong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hong, Seung-Beom
Lee, Mina
Kim, Dae-Ho
Varga, Janos
Frisvad, Jens C.
Perrone, Giancarlo
Gomi, Katsuya
Yamada, Osamu
Machida, Masayuki
Houbraken, Jos
Samson, Robert A.
ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA
title ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA
title_full ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA
title_fullStr ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA
title_full_unstemmed ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA
title_short ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA
title_sort aspergillus luchuensis , an industrially important black aspergillus in east asia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665839/
https://www.ncbi.nlm.nih.gov/pubmed/23723998
http://dx.doi.org/10.1371/journal.pone.0063769
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