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Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay
BACKGROUND: A crucial step in the evaluation of newly produced transgenic plants is the selection of homozygous plants. Here we describe an efficient and highly flexible real-time PCR-based method for the development of homozygous lines in plant models with complex (multiple) genomes and/or relative...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668147/ https://www.ncbi.nlm.nih.gov/pubmed/23627847 http://dx.doi.org/10.1186/1471-2229-13-71 |