Cargando…

Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay

BACKGROUND: A crucial step in the evaluation of newly produced transgenic plants is the selection of homozygous plants. Here we describe an efficient and highly flexible real-time PCR-based method for the development of homozygous lines in plant models with complex (multiple) genomes and/or relative...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mieog, Jos C, Howitt, Crispin A, Ral, Jean-Philippe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668147/ https://www.ncbi.nlm.nih.gov/pubmed/23627847 http://dx.doi.org/10.1186/1471-2229-13-71

Ejemplares similares

GrainScan: a low cost, fast method for grain size and colour measurements
por: Whan, Alex P, et al.
Publicado: (2014)

Fast and Efficient Screening for Wheat Loss-of-Gene Mutants Using Multiplexed Melt Curve Analyses
por: Mieog, Jos C., et al.
Publicado: (2016)

A heat-shock inducible system for flexible gene expression in cereals
por: Harrington, Sophie A., et al.
Publicado: (2020)

Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
por: Athman, Asmini, et al.
Publicado: (2014)

Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies
por: Sales, Kamila Gaudêncio da Silva, et al.
Publicado: (2020)

A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants
por: Guo, Jinggong, et al.
Publicado: (2018)

Identifying rapidly parasiticidal anti-malarial drugs using a simple and reliable in vitro parasite viability fast assay
por: Linares, María, et al.
Publicado: (2015)

Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
por: Wong, Samson S. Y., et al.
Publicado: (2018)

Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA
por: Paraskevis, Dimitrios, et al.
Publicado: (2010)

A simple quantitative PCR assay to determine TRAMP transgene zygosity
por: Chen, Ruidong, et al.
Publicado: (2020)

A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps
por: Atanassov, Ivan I, et al.
Publicado: (2009)

A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi
por: Divis, Paul CS, et al.
Publicado: (2010)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
por: Vezenegho, Samuel B, et al.
Publicado: (2009)

A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
por: Huang, Li, et al.
Publicado: (2015)

Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
por: Davó, Laura, et al.
Publicado: (2020)

Quantitative PCR for detection of the OT-1 transgene
por: Wright, Kate O, et al.
Publicado: (2005)

Fast-track transformation and genome editing in Brachypodium distachyon
por: Soulhat, Camille, et al.
Publicado: (2023)

Simple allele-discriminating PCR for cost-effective and rapid genotyping and mapping
por: Bui, Minh, et al.
Publicado: (2009)

Development and Validation of a HPV-32 Specific PCR Assay
por: Herrel, Nicholas R, et al.
Publicado: (2009)

Sample-ready multiplex qPCR assay for detection of malaria
por: Kamau, Edwin, et al.
Publicado: (2014)

Comparison of three PCR-based assays for SNP genotyping in plants
por: Broccanello, Chiara, et al.
Publicado: (2018)

Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
por: Safeukui, Innocent, et al.
Publicado: (2008)

Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus
por: Iwaki, Kosuke K, et al.
Publicado: (2010)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Evaluation of SARS-CoV-2 genome detection by real-time PCR assay using pooled specimens
por: Tavakoli, Forough, et al.
Publicado: (2022)

Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation
por: Faize, Mohamed, et al.
Publicado: (2010)

Real-time PCR assays for genotyping of Cryptococcus gattii in North America
por: Kelley, Erin J, et al.
Publicado: (2014)

Moving from qPCR to Chip Digital PCR Assays for Tracking of some Fusarium species causing Fusarium Head Blight in Cereals
por: Morcia, Caterina, et al.
Publicado: (2020)

A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
por: Tran, Tuan M, et al.
Publicado: (2014)

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
por: Qiu, Fang-zhou, et al.
Publicado: (2018)

Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)
por: He, Shengnan, et al.
Publicado: (2020)

Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans
por: van Bergen, Kim, et al.
Publicado: (2021)

Simple and rapid determination of homozygous transgenic mice via in vivo fluorescence imaging
por: Lin, Xiaolin, et al.
Publicado: (2015)

Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR
por: Bubner, Ben, et al.
Publicado: (2004)

Engineering α-amylase levels in wheat grain suggests a highly sophisticated level of carbohydrate regulation during development
por: Whan, Alex, et al.
Publicado: (2014)

Simple, sensitive and quantitative bioluminescence assay for determination of malaria pre-patent period
por: Zuzarte-Luis, Vanessa, et al.
Publicado: (2014)

Barley callus: a model system for bioengineering of starch in cereals
por: Carciofi, Massimiliano, et al.
Publicado: (2012)

A multiplex PCR assay for six Aedini species, including Aedes albopictus
por: Bang, Woo Jun, et al.
Publicado: (2021)

PCR–RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras
por: Escobar, Denis, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_8ap037htjgb5ot0db28k98q7n4