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Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing

Transcript leaders (TLs) can have profound effects on mRNA translation and stability. To map TL boundaries genome-wide, we developed TL-sequencing (TL-seq), a technique combining enzymatic capture of m(7)G-capped mRNA 5′ ends with high-throughput sequencing. TL-seq identified mRNA start sites for th...

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Detalles Bibliográficos
Autores principales: Arribere, Joshua A., Gilbert, Wendy V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668365/
https://www.ncbi.nlm.nih.gov/pubmed/23580730
http://dx.doi.org/10.1101/gr.150342.112