Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()

EphA2 is activated through phosphorylation on serine 897 (S897) by Akt to promote cancer cell motility and invasion, independently of stimulation by ephrin, its ligand. Here we show that S897 phosphorylation of EphA2 strengthens the interaction between EphA2 and Ephexin4, a guanine nucleotide exchan...

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Detalles Bibliográficos
Autores principales: Kawai, Hiromu, Kobayashi, Masakazu, Hiramoto-Yamaki, Nao, Harada, Kohei, Negishi, Manabu, Katoh, Hironori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668535/
https://www.ncbi.nlm.nih.gov/pubmed/23772378
http://dx.doi.org/10.1016/j.fob.2013.01.002
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author Kawai, Hiromu
Kobayashi, Masakazu
Hiramoto-Yamaki, Nao
Harada, Kohei
Negishi, Manabu
Katoh, Hironori
author_facet Kawai, Hiromu
Kobayashi, Masakazu
Hiramoto-Yamaki, Nao
Harada, Kohei
Negishi, Manabu
Katoh, Hironori
author_sort Kawai, Hiromu
collection PubMed
description EphA2 is activated through phosphorylation on serine 897 (S897) by Akt to promote cancer cell motility and invasion, independently of stimulation by ephrin, its ligand. Here we show that S897 phosphorylation of EphA2 strengthens the interaction between EphA2 and Ephexin4, a guanine nucleotide exchange factor for the small GTPase RhoG. S897A mutation of EphA2 abolished the EphA2/Ephexin4-mediated RhoG activation, promotion of cell migration, and resistance to anoikis. Our results suggest that S897-phosphorylated EphA2 recruits Ephexin4 to promote cell migration and anoikis resistance, providing a molecular link between S897 phosphorylation of EphA2 and tumor progression.
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spelling pubmed-36685352013-06-14 Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2() Kawai, Hiromu Kobayashi, Masakazu Hiramoto-Yamaki, Nao Harada, Kohei Negishi, Manabu Katoh, Hironori FEBS Open Bio Article EphA2 is activated through phosphorylation on serine 897 (S897) by Akt to promote cancer cell motility and invasion, independently of stimulation by ephrin, its ligand. Here we show that S897 phosphorylation of EphA2 strengthens the interaction between EphA2 and Ephexin4, a guanine nucleotide exchange factor for the small GTPase RhoG. S897A mutation of EphA2 abolished the EphA2/Ephexin4-mediated RhoG activation, promotion of cell migration, and resistance to anoikis. Our results suggest that S897-phosphorylated EphA2 recruits Ephexin4 to promote cell migration and anoikis resistance, providing a molecular link between S897 phosphorylation of EphA2 and tumor progression. Elsevier 2013-01-21 /pmc/articles/PMC3668535/ /pubmed/23772378 http://dx.doi.org/10.1016/j.fob.2013.01.002 Text en © 2013 The Authors http://creativecommons.org/licenses/BY-NC-ND/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Kawai, Hiromu
Kobayashi, Masakazu
Hiramoto-Yamaki, Nao
Harada, Kohei
Negishi, Manabu
Katoh, Hironori
Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()
title Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()
title_full Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()
title_fullStr Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()
title_full_unstemmed Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()
title_short Ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of EphA2()
title_sort ephexin4-mediated promotion of cell migration and anoikis resistance is regulated by serine 897 phosphorylation of epha2()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668535/
https://www.ncbi.nlm.nih.gov/pubmed/23772378
http://dx.doi.org/10.1016/j.fob.2013.01.002
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