A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences

BACKGROUND: In vivo recombination of overlapping DNA fragments for assembly of large DNA constructs in the yeast Saccharomyces cerevisiae holds great potential for pathway engineering on a small laboratory scale as well as for automated high-throughput strain construction. However, the current in vi...

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Detalles Bibliográficos
Autores principales: Kuijpers, Niels GA, Solis-Escalante, Daniel, Bosman, Lizanne, van den Broek, Marcel, Pronk, Jack T, Daran, Jean-Marc, Daran-Lapujade, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669052/
https://www.ncbi.nlm.nih.gov/pubmed/23663359
http://dx.doi.org/10.1186/1475-2859-12-47

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669052/
https://www.ncbi.nlm.nih.gov/pubmed/23663359
http://dx.doi.org/10.1186/1475-2859-12-47

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