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Malolactic enzyme from Oenococcus oeni: Heterologous expression in Escherichia coli and biochemical characterization

Malolactic enzymes (MLE) are known to directly convert L-malic acid into L-lactic acid with a catalytical requirement of nicotinamide adenine dinucleotide (NAD(+)) and Mn(2+); however, the reaction mechanism is still unclear. To study a MLE, the structural gene from Oenococcus oeni strain DSM 20255...

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Detalles Bibliográficos
Autores principales: Schümann, Christina, Michlmayr, Herbert, del Hierro, Andrés M., Kulbe, Klaus D., Jiranek, Vladimir, Eder, Reinhard, Nguyen, Thu-Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669155/
https://www.ncbi.nlm.nih.gov/pubmed/23196745
http://dx.doi.org/10.4161/bioe.22988