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REXO2 Is an Oligoribonuclease Active in Human Mitochondria

The Escherichia coli oligoribonuclease, ORN, has a 3′ to 5′ exonuclease activity specific for small oligomers that is essential for cell viability. The human homologue, REXO2, has hitherto been incompletely characterized, with only its in vitro ability to degrade small single-stranded RNA and DNA fr...

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Autores principales: Bruni, Francesco, Gramegna, Pasqua, Oliveira, Jorge M. A., Lightowlers, Robert N., Chrzanowska-Lightowlers, Zofia M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669425/
https://www.ncbi.nlm.nih.gov/pubmed/23741365
http://dx.doi.org/10.1371/journal.pone.0064670
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author Bruni, Francesco
Gramegna, Pasqua
Oliveira, Jorge M. A.
Lightowlers, Robert N.
Chrzanowska-Lightowlers, Zofia M. A.
author_facet Bruni, Francesco
Gramegna, Pasqua
Oliveira, Jorge M. A.
Lightowlers, Robert N.
Chrzanowska-Lightowlers, Zofia M. A.
author_sort Bruni, Francesco
collection PubMed
description The Escherichia coli oligoribonuclease, ORN, has a 3′ to 5′ exonuclease activity specific for small oligomers that is essential for cell viability. The human homologue, REXO2, has hitherto been incompletely characterized, with only its in vitro ability to degrade small single-stranded RNA and DNA fragments documented. Here we show that the human enzyme has clear dual cellular localization being present both in cytosolic and mitochondrial fractions. Interestingly, the mitochondrial form localizes to both the intermembrane space and the matrix. Depletion of REXO2 by RNA interference causes a strong morphological phenotype in human cells, which show a disorganized network of punctate and granular mitochondria. Lack of REXO2 protein also causes a substantial decrease of mitochondrial nucleic acid content and impaired de novo mitochondrial protein synthesis. Our data constitute the first in vivo evidence for an oligoribonuclease activity in human mitochondria.
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spelling pubmed-36694252013-06-05 REXO2 Is an Oligoribonuclease Active in Human Mitochondria Bruni, Francesco Gramegna, Pasqua Oliveira, Jorge M. A. Lightowlers, Robert N. Chrzanowska-Lightowlers, Zofia M. A. PLoS One Research Article The Escherichia coli oligoribonuclease, ORN, has a 3′ to 5′ exonuclease activity specific for small oligomers that is essential for cell viability. The human homologue, REXO2, has hitherto been incompletely characterized, with only its in vitro ability to degrade small single-stranded RNA and DNA fragments documented. Here we show that the human enzyme has clear dual cellular localization being present both in cytosolic and mitochondrial fractions. Interestingly, the mitochondrial form localizes to both the intermembrane space and the matrix. Depletion of REXO2 by RNA interference causes a strong morphological phenotype in human cells, which show a disorganized network of punctate and granular mitochondria. Lack of REXO2 protein also causes a substantial decrease of mitochondrial nucleic acid content and impaired de novo mitochondrial protein synthesis. Our data constitute the first in vivo evidence for an oligoribonuclease activity in human mitochondria. Public Library of Science 2013-05-31 /pmc/articles/PMC3669425/ /pubmed/23741365 http://dx.doi.org/10.1371/journal.pone.0064670 Text en © 2013 Bruni et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bruni, Francesco
Gramegna, Pasqua
Oliveira, Jorge M. A.
Lightowlers, Robert N.
Chrzanowska-Lightowlers, Zofia M. A.
REXO2 Is an Oligoribonuclease Active in Human Mitochondria
title REXO2 Is an Oligoribonuclease Active in Human Mitochondria
title_full REXO2 Is an Oligoribonuclease Active in Human Mitochondria
title_fullStr REXO2 Is an Oligoribonuclease Active in Human Mitochondria
title_full_unstemmed REXO2 Is an Oligoribonuclease Active in Human Mitochondria
title_short REXO2 Is an Oligoribonuclease Active in Human Mitochondria
title_sort rexo2 is an oligoribonuclease active in human mitochondria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669425/
https://www.ncbi.nlm.nih.gov/pubmed/23741365
http://dx.doi.org/10.1371/journal.pone.0064670
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