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Densitometry based microassay for the determination of lipase depolymerizing activity on polyhydroxyalkanoate

A novel method for the assay of polyhydroxyalkanoate (PHA)-degrading ability of triacylglycerol lipases was developed. By applying the natural affinity of lipases towards hydrophobic interfaces, a sensitive and rapid densitometry analysis for the evaluation of hydrolytic activity of lipase droplets...

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Detalles Bibliográficos
Autores principales: Ch’ng, Diana Hooi-Ean, Sudesh, Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671206/
https://www.ncbi.nlm.nih.gov/pubmed/23657221
http://dx.doi.org/10.1186/2191-0855-3-22