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SOMA: A Single Oligonucleotide Mutagenesis and Cloning Approach
Modern biology research requires simple techniques for efficient and restriction site-independent modification of genetic material. Classical cloning and mutagenesis strategies are limited by their dependency on restriction sites and the use of complementary primer pairs. Here, we describe the Singl...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672168/ https://www.ncbi.nlm.nih.gov/pubmed/23750217 http://dx.doi.org/10.1371/journal.pone.0064870 |