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SOMA: A Single Oligonucleotide Mutagenesis and Cloning Approach

Modern biology research requires simple techniques for efficient and restriction site-independent modification of genetic material. Classical cloning and mutagenesis strategies are limited by their dependency on restriction sites and the use of complementary primer pairs. Here, we describe the Singl...

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Detalles Bibliográficos
Autores principales: Pfirrmann, Thorsten, Lokapally, Ashwin, Andréasson, Claes, Ljungdahl, Per, Hollemann, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672168/
https://www.ncbi.nlm.nih.gov/pubmed/23750217
http://dx.doi.org/10.1371/journal.pone.0064870