Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system

Murine 3T3-L1 adipocytes are widely used as a cellular model of obesity. However, whereas transfection of 3T3-L1 preadipocytes is straightforward, ectopic gene expression in mature 3T3-L1 adipocytes has proved challenging. Here, we used the pSLIK vector system to generate stable doxycycline-inducibl...

Descripción completa

Detalles Bibliográficos
Autores principales: Esteves, Cristina L, Kelly, Val, Bégay, Valérie, Lillico, Simon G, Leutz, Achim, Seckl, Jonathan R, Chapman, Karen E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Endocrinology 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672996/
https://www.ncbi.nlm.nih.gov/pubmed/23620165
http://dx.doi.org/10.1530/JME-13-0029

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672996/
https://www.ncbi.nlm.nih.gov/pubmed/23620165
http://dx.doi.org/10.1530/JME-13-0029

Ejemplares similares