Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

We describe a new cell-free protein synthesis (CFPS) method for site-specific incorporation of non-natural amino acids (nnAAs) into proteins in which the orthogonal tRNA (o-tRNA) and the modified protein (i.e. the protein containing the nnAA) are produced simultaneously. Using this method, 0.9–1.7 m...

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Detalles Bibliográficos
Autores principales: Albayrak, Cem, Swartz, James R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Synthetic Biology and Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675464/
https://www.ncbi.nlm.nih.gov/pubmed/23589624
http://dx.doi.org/10.1093/nar/gkt226

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675464/
https://www.ncbi.nlm.nih.gov/pubmed/23589624
http://dx.doi.org/10.1093/nar/gkt226

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