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Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation
We describe a new cell-free protein synthesis (CFPS) method for site-specific incorporation of non-natural amino acids (nnAAs) into proteins in which the orthogonal tRNA (o-tRNA) and the modified protein (i.e. the protein containing the nnAA) are produced simultaneously. Using this method, 0.9–1.7 m...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675464/ https://www.ncbi.nlm.nih.gov/pubmed/23589624 http://dx.doi.org/10.1093/nar/gkt226 |