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Massively parallel characterization of restriction endonucleases

Restriction endonucleases are highly specific in recognizing the particular DNA sequence they act on. However, their activity is affected by sequence context, enzyme concentration and buffer composition. Changes in these factors may lead to either ineffective cleavage at the cognate restriction site...

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Detalles Bibliográficos
Autores principales: Kamps-Hughes, Nick, Quimby, Aine, Zhu, Zhenyu, Johnson, Eric A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675476/
https://www.ncbi.nlm.nih.gov/pubmed/23605040
http://dx.doi.org/10.1093/nar/gkt257