Cargando…
Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901
Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676750/ https://www.ncbi.nlm.nih.gov/pubmed/23615464 http://dx.doi.org/10.3390/ijms14058684 |
_version_ | 1782272656938106880 |
---|---|
author | Zhu, Xiaoyan Luo, Fenglei Zheng, Yixiong Zhang, Jiukai Huang, Jianzhen Sun, Chongde Li, Xian Chen, Kunsong |
author_facet | Zhu, Xiaoyan Luo, Fenglei Zheng, Yixiong Zhang, Jiukai Huang, Jianzhen Sun, Chongde Li, Xian Chen, Kunsong |
author_sort | Zhu, Xiaoyan |
collection | PubMed |
description | Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. |
format | Online Article Text |
id | pubmed-3676750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36767502013-07-02 Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 Zhu, Xiaoyan Luo, Fenglei Zheng, Yixiong Zhang, Jiukai Huang, Jianzhen Sun, Chongde Li, Xian Chen, Kunsong Int J Mol Sci Article Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. Molecular Diversity Preservation International (MDPI) 2013-04-24 /pmc/articles/PMC3676750/ /pubmed/23615464 http://dx.doi.org/10.3390/ijms14058684 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Zhu, Xiaoyan Luo, Fenglei Zheng, Yixiong Zhang, Jiukai Huang, Jianzhen Sun, Chongde Li, Xian Chen, Kunsong Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 |
title | Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 |
title_full | Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 |
title_fullStr | Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 |
title_full_unstemmed | Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 |
title_short | Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901 |
title_sort | characterization, purification of poncirin from edible citrus ougan (citrus reticulate cv. suavissima) and its growth inhibitory effect on human gastric cancer cells sgc-7901 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676750/ https://www.ncbi.nlm.nih.gov/pubmed/23615464 http://dx.doi.org/10.3390/ijms14058684 |
work_keys_str_mv | AT zhuxiaoyan characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT luofenglei characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT zhengyixiong characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT zhangjiukai characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT huangjianzhen characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT sunchongde characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT lixian characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 AT chenkunsong characterizationpurificationofponcirinfromediblecitrusougancitrusreticulatecvsuavissimaanditsgrowthinhibitoryeffectonhumangastriccancercellssgc7901 |