Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676757/ https://www.ncbi.nlm.nih.gov/pubmed/23615471 http://dx.doi.org/10.3390/ijms14058801 |
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author | Kuo, Ying-Yu Lin, Hui-Ping Huo, Chieh Su, Liang-Cheng Yang, Jonathan Hsiao, Ping-Hsuan Chiang, Hung-Che Chung, Chi-Jung Wang, Horng-Dar Chang, Jang-Yang Chen, Ya-Wen Chuu, Chih-Pin |
author_facet | Kuo, Ying-Yu Lin, Hui-Ping Huo, Chieh Su, Liang-Cheng Yang, Jonathan Hsiao, Ping-Hsuan Chiang, Hung-Che Chung, Chi-Jung Wang, Horng-Dar Chang, Jang-Yang Chen, Ya-Wen Chuu, Chih-Pin |
author_sort | Kuo, Ying-Yu |
collection | PubMed |
description | Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27(Kip). Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer. |
format | Online Article Text |
id | pubmed-3676757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36767572013-07-02 Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling Kuo, Ying-Yu Lin, Hui-Ping Huo, Chieh Su, Liang-Cheng Yang, Jonathan Hsiao, Ping-Hsuan Chiang, Hung-Che Chung, Chi-Jung Wang, Horng-Dar Chang, Jang-Yang Chen, Ya-Wen Chuu, Chih-Pin Int J Mol Sci Article Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27(Kip). Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer. Molecular Diversity Preservation International (MDPI) 2013-04-24 /pmc/articles/PMC3676757/ /pubmed/23615471 http://dx.doi.org/10.3390/ijms14058801 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kuo, Ying-Yu Lin, Hui-Ping Huo, Chieh Su, Liang-Cheng Yang, Jonathan Hsiao, Ping-Hsuan Chiang, Hung-Che Chung, Chi-Jung Wang, Horng-Dar Chang, Jang-Yang Chen, Ya-Wen Chuu, Chih-Pin Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling |
title | Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling |
title_full | Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling |
title_fullStr | Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling |
title_full_unstemmed | Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling |
title_short | Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling |
title_sort | caffeic acid phenethyl ester suppresses proliferation and survival of tw2.6 human oral cancer cells via inhibition of akt signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676757/ https://www.ncbi.nlm.nih.gov/pubmed/23615471 http://dx.doi.org/10.3390/ijms14058801 |
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