Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents

Several rhein-phosphonate derivatives (5a–c) were synthesized and evaluated for in vitro cytotoxicity against HepG-2, CNE, Spca-2, Hela and Hct-116 cell lines. Some compounds showed relatively high cytotoxicity. Especially compounds 5b exhibited the strongest cytotoxicity against HepG-2 and Spca-2 c...

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Detalles Bibliográficos
Autores principales: Ye, Man-Yi, Yao, Gui-Yang, Wei, Jing-Chen, Pan, Ying-Ming, Liao, Zhi-Xin, Wang, Heng-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676791/
https://www.ncbi.nlm.nih.gov/pubmed/23629673
http://dx.doi.org/10.3390/ijms14059424
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author Ye, Man-Yi
Yao, Gui-Yang
Wei, Jing-Chen
Pan, Ying-Ming
Liao, Zhi-Xin
Wang, Heng-Shan
author_facet Ye, Man-Yi
Yao, Gui-Yang
Wei, Jing-Chen
Pan, Ying-Ming
Liao, Zhi-Xin
Wang, Heng-Shan
author_sort Ye, Man-Yi
collection PubMed
description Several rhein-phosphonate derivatives (5a–c) were synthesized and evaluated for in vitro cytotoxicity against HepG-2, CNE, Spca-2, Hela and Hct-116 cell lines. Some compounds showed relatively high cytotoxicity. Especially compounds 5b exhibited the strongest cytotoxicity against HepG-2 and Spca-2 cells (IC(50) was 8.82 and 9.01 μM), respectively. All the synthesized compounds exhibited low cytotoxicity against HUVEC cells. Further experiments proved that 5b could disturb the cell cycle in HepG-2 cells and induce apoptosis. In addition, the binding properties of a model conjugate 5b to DNA were investigated by methods (UV-Vis, fluorescence, CD spectroscopy). Results indicated that 5b showed moderate ability to interact ct-DNA.
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spelling pubmed-36767912013-07-02 Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents Ye, Man-Yi Yao, Gui-Yang Wei, Jing-Chen Pan, Ying-Ming Liao, Zhi-Xin Wang, Heng-Shan Int J Mol Sci Article Several rhein-phosphonate derivatives (5a–c) were synthesized and evaluated for in vitro cytotoxicity against HepG-2, CNE, Spca-2, Hela and Hct-116 cell lines. Some compounds showed relatively high cytotoxicity. Especially compounds 5b exhibited the strongest cytotoxicity against HepG-2 and Spca-2 cells (IC(50) was 8.82 and 9.01 μM), respectively. All the synthesized compounds exhibited low cytotoxicity against HUVEC cells. Further experiments proved that 5b could disturb the cell cycle in HepG-2 cells and induce apoptosis. In addition, the binding properties of a model conjugate 5b to DNA were investigated by methods (UV-Vis, fluorescence, CD spectroscopy). Results indicated that 5b showed moderate ability to interact ct-DNA. Molecular Diversity Preservation International (MDPI) 2013-04-29 /pmc/articles/PMC3676791/ /pubmed/23629673 http://dx.doi.org/10.3390/ijms14059424 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ye, Man-Yi
Yao, Gui-Yang
Wei, Jing-Chen
Pan, Ying-Ming
Liao, Zhi-Xin
Wang, Heng-Shan
Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents
title Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents
title_full Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents
title_fullStr Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents
title_full_unstemmed Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents
title_short Synthesis, Cytotoxicity, DNA Binding and Apoptosis of Rhein-Phosphonate Derivatives as Antitumor Agents
title_sort synthesis, cytotoxicity, dna binding and apoptosis of rhein-phosphonate derivatives as antitumor agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676791/
https://www.ncbi.nlm.nih.gov/pubmed/23629673
http://dx.doi.org/10.3390/ijms14059424
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