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Experimental evidence for the involvement of amino acid residue Glu398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase

The role of glutamate 398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase (BlGGT) was explored by site-directed mutagenesis. This glutamate was substituted by either alanine, aspartate, arginine or glutamine and the expressed mutant enzymes were purified to apparen...

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Detalles Bibliográficos
Autores principales: Chi, Meng-Chun, Chen, Yi-Yu, Lo, Huei-Fen, Lin, Long-Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678132/
https://www.ncbi.nlm.nih.gov/pubmed/23772362
http://dx.doi.org/10.1016/j.fob.2012.09.007