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Experimental evidence for the involvement of amino acid residue Glu398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase
The role of glutamate 398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase (BlGGT) was explored by site-directed mutagenesis. This glutamate was substituted by either alanine, aspartate, arginine or glutamine and the expressed mutant enzymes were purified to apparen...
Autores principales: | Chi, Meng-Chun, Chen, Yi-Yu, Lo, Huei-Fen, Lin, Long-Liu |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678132/ https://www.ncbi.nlm.nih.gov/pubmed/23772362 http://dx.doi.org/10.1016/j.fob.2012.09.007 |
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