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Increasing the library size in cDNA display by optimizing purification procedures
BACKGROUND: The library size is critical for selection in evolutionary molecular engineering (directed evolution). Although cDNA display has become a promising in vitro display technology by overcoming the instability of mRNA display, it is hindered by low yields. In this study, we improved the yiel...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680162/ https://www.ncbi.nlm.nih.gov/pubmed/23697943 http://dx.doi.org/10.1186/1480-9222-15-7 |